diff --git a/tests/test_python.py b/tests/test_python.py
index 7b6c6f5ae98..dff14994112 100644
--- a/tests/test_python.py
+++ b/tests/test_python.py
@@ -49,6 +49,16 @@ def test_predict_dir():
     model(source=ASSETS, imgsz=32)
 
 
+def test_predict_txt():
+    # Write a list of sources to a txt file
+    txt_file = TMP / 'sources.txt'
+    with open(txt_file, 'w') as f:
+        for x in [ASSETS / 'bus.jpg', ASSETS / 'zidane.jpg']:
+            f.write(f'{x}\n')
+    model = YOLO(MODEL)
+    model(source=txt_file, imgsz=640)
+
+
 def test_predict_img():
     model = YOLO(MODEL)
     seg_model = YOLO(WEIGHTS_DIR / 'yolov8n-seg.pt')
diff --git a/ultralytics/data/loaders.py b/ultralytics/data/loaders.py
index 1534a50003c..cdbf5e4c5bc 100644
--- a/ultralytics/data/loaders.py
+++ b/ultralytics/data/loaders.py
@@ -182,7 +182,7 @@ def __init__(self, path, imgsz=640, vid_stride=1):
         parent = None
         if isinstance(path, str) and Path(path).suffix == '.txt':  # *.txt file with img/vid/dir on each line
             parent = Path(path).parent
-            path = Path(path).read_text().rsplit()
+            path = Path(path).read_text().splitlines()  # list of sources
         files = []
         for p in sorted(path) if isinstance(path, (list, tuple)) else [path]:
             a = str(Path(p).absolute())  # do not use .resolve() https://github.com/ultralytics/ultralytics/issues/2912