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Description:
I am encountering contamination scores ranging from 6-9% for the following species:
Blautia sp001304935
Enterocloster lavalensis
Enterocloster citroniae
Enterocloster clostridioformis
Hungatella sp005845265
Hungatella effluvii
The scores consistently fall within a 0.5% difference range between multiple isolates, even when obtained from different sources, such as the NCBI references.
Observations:
When analyzed using Metaphlan4, the genomes are tagged as 100% matching the species identified by GTDBK.
Consistent scores are observed between isolates of the same species.
Certain genera seem to contain multiple of these offenders.
Suspicion:
Given the alignment of results between isolates, the 100% match with Metaphlan4, and the recurring appearance of specific genera, there is a suspicion that there might be no actual contamination from other species or strains within the same species and that there is some set of genes or traits within these species causing this to flag up.
I'm not sure what the right approach for dealing with this is, but I thought I should put a notice out for it.
The text was updated successfully, but these errors were encountered:
Description:
I am encountering contamination scores ranging from 6-9% for the following species:
The scores consistently fall within a 0.5% difference range between multiple isolates, even when obtained from different sources, such as the NCBI references.
Observations:
When analyzed using Metaphlan4, the genomes are tagged as 100% matching the species identified by GTDBK.
Consistent scores are observed between isolates of the same species.
Certain genera seem to contain multiple of these offenders.
Suspicion:
Given the alignment of results between isolates, the 100% match with Metaphlan4, and the recurring appearance of specific genera, there is a suspicion that there might be no actual contamination from other species or strains within the same species and that there is some set of genes or traits within these species causing this to flag up.
I'm not sure what the right approach for dealing with this is, but I thought I should put a notice out for it.
The text was updated successfully, but these errors were encountered: