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protein_seq_for_snv_using_refseq_bed_orig.pl
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protein_seq_for_snv_using_refseq_bed_orig.pl
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#
use strict;
#!/usr/bin/perl
## generate mutant and wild-type protein sequence for snv ##
## 12-01-2016 ##
use strict;
(my $usage = <<OUT) =~ s/\t+//g;
This script will get the protein sequence for both wide-type and mutants
OUT
my $error=0;
die $usage unless @ARGV == 3;
my($filename,$dir,$filename_vcf_somatic)=@ARGV;
#if ($ARGV[0]=~/\w/) { $filename=$ARGV[0];} else { $error=1; }
#if ($ARGV[1]=~/\w/) { $dir=$ARGV[1];} else { $dir="."; }
#if ($ARGV[2]=~/\w/) { $filename_vcf_germline=$ARGV[2];} else { $filename_vcf_germline=""; }
#if ($ARGV[3]=~/\w/) { $filename_vcf_somatic=$ARGV[3];} else { $filename_vcf_somatic=""; }
my %mapping = ( "TTT"=>"F","TTC"=>"F","TTA"=>"L","TTG"=>"L",
"CTT"=>"L","CTC"=>"L","CTA"=>"L","CTG"=>"L",
"ATT"=>"I","ATC"=>"I","ATA"=>"I","ATG"=>"M",
"GTT"=>"V","GTC"=>"V","GTA"=>"V","GTG"=>"V",
"TCT"=>"S","TCC"=>"S","TCA"=>"S","TCG"=>"S",
"CCT"=>"P","CCC"=>"P","CCA"=>"P","CCG"=>"P",
"ACT"=>"T","ACC"=>"T","ACA"=>"T","ACG"=>"T",
"GCT"=>"A","GCC"=>"A","GCA"=>"A","GCG"=>"A",
"TAT"=>"Y","TAC"=>"Y","TAA"=>"*","TAG"=>"*",
"CAT"=>"H","CAC"=>"H","CAA"=>"Q","CAG"=>"Q",
"AAT"=>"N","AAC"=>"N","AAA"=>"K","AAG"=>"K",
"GAT"=>"D","GAC"=>"D","GAA"=>"E","GAG"=>"E",
"TGT"=>"C","TGC"=>"C","TGA"=>"*","TGG"=>"W",
"CGT"=>"R","CGC"=>"R","CGA"=>"R","CGG"=>"R",
"AGT"=>"S","AGC"=>"S","AGA"=>"R","AGG"=>"R",
"GGT"=>"G","GGC"=>"G","GGA"=>"G","GGG"=>"G");
if ($error==0)
{
my $filename_=$filename;
$filename_=~s/\.bed$//;
open (OUT,">$filename_vcf_somatic.proteome-snv-wt.fasta");
open (OUT_MOD,">$filename_vcf_somatic.proteome-snv-mut.fasta");
open (OUT_FS,">$filename_vcf_somatic.proteome-snv-fs.fasta");
open (LOG,">$filename_vcf_somatic.proteome-snv-wt.log");
open (LOG_MOD,">$filename_vcf_somatic.proteome-snv-mut.log");
open (STAT,">$filename_vcf_somatic.proteome-snv-mod.stat");
my $proteins_count=0;
my $proteins_modified=0;
my $count_stop_removed=0;
my $count_stop_introduced=0;
my $count_stop_removed_somatic=0;
my $count_stop_introduced_somatic=0;
my $protein_modifications_count=0;
my @protein_modifications_distr=();
my $protein_modifications_count_somatic=0;
my @protein_modifications_distr_somatic=();
my $count_variant_in_exon=0;
my $count_variant_in_exon_somatic=0;
my $count_variant_in_exon_old_error=0;
my $count_variant_in_exon_old_error_somatic=0;
my $count_variant_in_exon_nonsyn=0;
my $count_variant_in_exon_nonsyn_somatic=0;
my $line="";
my %chr=();
my %bed=();
my %seq=();
if (open (IN,"$filename"))
{
while ($line=<IN>)
{
chomp($line);
if ($line=~/^([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)$/)
{
my $chr=$1;
my $name=$4;
$bed{$name}=$line;
$chr{$chr}.="#$name#";
} else { print LOG qq!Error parsing: $line!; }
}
close(IN);
}
my %descriptions=();
if (open (IN,"$filename_-descriptions.txt"))
{
while ($line=<IN>)
{
chomp($line);
if ($line=~/^([^\t]+)\t([^\t]+)/)
{
$descriptions{$1}=$2;
}
}
close(IN);
}
my %vcf_old=();
my %vcf_new=();
my %vcf_type=();
my %vcf_anno=();
my %vcf_gene=();
my $germline_vcf=0;
my $germline_only=0;
my $somatic_only=0;
my $both_vcf=0;
my $both_vcf_differ=0;
# if (open (IN,"$filename_vcf_germline"))
# {
# while ($line=<IN>)
# {
# chomp($line);
# if ($line=~/^([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)/)
# {
# my $chr="chr$1";
# my $pos=$2;
# my $id=$3;
# my $old=$4;
# my $new=$5;
# my $qaul=$6;
# $pos--;
# $new=~s/\,.*$//; #####
# $vcf_old{"$chr#$pos"}=$old;
# $vcf_new{"$chr#$pos"}=$new;
# $vcf_type{"$chr#$pos"}="G";
# $germline_vcf++;
#print qq!$chr#$pos#$new\n!;
# } else { print LOG_MOD qq!Error parsing: $line!; }
# }
# close(IN);
# }
# if (open (IN,"$filename_vcf_somatic"))
# {
#open (OUT_ONLY,">$filename_vcf_somatic-somatic_only.vcf");
# while ($line=<IN>)
# {
# chomp($line);
# if ($line=~/^([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)/)
# {
# my $chr="chr$1";
# my $pos=$2;
# my $id=$3;
# my $old=$4;
# my $new=$5;
# my $qaul=$6;
# $pos--;
# $new=~s/\,.*$//; #####
# if ($vcf_old{"$chr#$pos"}=~/\w/)
# {
# if ($vcf_new{"$chr#$pos"}!~/^$new$/)
# {
#print LOG_MOD qq!$chr $pos: germline:$vcf_old{"$chr#$pos"}->$vcf_new{"$chr#$pos"} somatic:$old->$new\n!;
# $vcf_old{"$chr#$pos"}=$old;
# $vcf_new{"$chr#$pos"}=$new;
# $vcf_type{"$chr#$pos"}="S";
#print OUT_ONLY qq!$line\n!;
# $somatic_only++;
# $both_vcf_differ++;
# }
# $both_vcf++;
# }
# else
# {
# $vcf_old{"$chr#$pos"}=$old;
# $vcf_new{"$chr#$pos"}=$new;
# $vcf_type{"$chr#$pos"}="S";
#print OUT_ONLY qq!$line\n!;
# $somatic_only++;
# }
#print qq!$chr#$pos#$new\n!;
# } else { print LOG_MOD qq!Error parsing: $line!; }
# }
# close(IN);
#close(OUT_ONLY);
# }
# $germline_only=$germline_vcf-$both_vcf_differ;
# print qq!Somatic: $somatic_only\nGermline: $germline_only\nIn both VCF: $both_vcf (differ: $both_vcf_differ)\n!;
if(!-e $filename_vcf_somatic)
{
print STAT "no vcf file\n";
last;
}
open (IN,"<$filename_vcf_somatic");
while ($line=<IN>)
{
chomp($line);
print $line,"\n";
my @temp=split("\t",$line);
my $chr="chr".$temp[0];
my $pos=$temp[1];
my $old=$temp[2];
my $new=$temp[3];
my $gene=$temp[4];
my $anno=$temp[5];
my $type=$temp[6];
$pos--;
$new=~s/\,.*$//; #####
print "pos=$pos","\t","old=$old", "\t", "new=$new","\t","anno=$anno","\t","type=$type","\n";
#<STDIN>;
#if ($vcf_old{"$chr#$pos"}=~/\w/)
#{
# if ($vcf_new{"$chr#$pos"}!~/^$new$/)
# {
#print LOG_MOD qq!$chr $pos: germline:$vcf_old{"$chr#$pos"}->$vcf_new{"$chr#$pos"} somatic:$old->$new\n!;
$vcf_old{"$chr#$pos"}=$old;
$vcf_new{"$chr#$pos"}=$new;
if($type eq "SOMATIC")
{ $vcf_type{"$chr#$pos"}="S";}
else { $vcf_type{"$chr#$pos"}="G"; }
$vcf_anno{"$chr#$pos"}=$anno;
#print OUT_ONLY qq!$line\n!;
$somatic_only++;
$vcf_gene{"$chr#$pos"}=$gene;
}
close(IN);
foreach my $chr (sort keys %chr)
{
print qq!$chr\n!;
if (open (IN,"$dir/$chr.fa"))
{
print qq!opened $chr\n!;
my $sequence="";
$line=<IN>;
chomp($line);
my $chr_=$chr;
$chr_=~s/^chr//i;
if ($line=~/^>$chr\s*/ or $line=~/^>$chr_\s*/)
{
while ($line=<IN>)
{
chomp($line);
if ($line=~/^>/)
{
print LOG qq!Error: > not expected: $line\n!;
}
else
{
$line=~s/\s+//g;
if ($line!~/^[atcgATCGnN]+$/)
{
print LOG qq!Error: unexpected character: $line\n!;
}
else
{
$sequence .= "\U$line";
}
}
}
my $temp=$chr{$chr};
while ($temp=~s/^#([^#]+)#//)
{
my $name=$1;
my $modified=0;
#print LOG qq!\n$name: $bed{$name}\n!;
if ($bed{$name}=~/^([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)\t([^\t]*)$/)
{
my $start=$2;
my $end=$3;
my $strand=$6;
my $num=$10;
my $segment_lengths="$11,";
my $segment_starts="$12,";
my $segment_lengths_extended="$11,";
my $segment_starts_extended="$12,";
if ($strand=~/\+/) { if ($segment_lengths_extended=~s/([0-9]+)\,$//) { my $temp=$1; $temp+=100; $segment_lengths_extended.="$temp,"; } }
else
{
if ($segment_lengths_extended=~s/^([0-9]+)\,//) { my $temp=$1; $temp+=100; $segment_lengths_extended="$temp,$segment_lengths_extended,"; }
if ($segment_starts_extended=~s/^([0-9]+)\,//) { my $temp=$1; $temp-=100; $segment_starts_extended="$temp,$segment_starts_extended,"; }
}
# print LOG qq!$segment_lengths $segment_lengths_extended $segment_starts $segment_starts_extended\n!;
my $seq_original="";
my $segment_starts_=$segment_starts;
my $segment_lengths_=$segment_lengths;
while ($segment_starts_=~s/^([0-9\-]+)\,//)
{
my $segment_start=$1;
if ($segment_lengths_=~s/^([0-9]+)\,//)
{
my $segment_length=$1;
my $seq_=substr $sequence,$start+$segment_start,$segment_length;
$seq_original.=$seq_;
# print LOG qq!$start+$segment_start,$segment_length: $seq_\n!;
} else { print LOG qq!Error parsing $bed{$name}\n!; }
}
my $seq="";
my $description_="(VAR:";
my %variants=();
my %variants_=();
my %variants2=();
my %variants2_=();
my $variant_loc="";
my $seqment_lengths_sum=0;
my $seqment_count=0;
my $segment_start_first=0;
$segment_start_first=0;
#$segment_starts_=$segment_starts_extended;
#$segment_lengths_=$segment_lengths_extended;
$segment_starts_=$segment_starts;
$segment_lengths_=$segment_lengths;
if ($segment_starts_=~/^([0-9\-]+)\,/) { $segment_start_first=$1; }
while ($segment_starts_=~s/^([0-9\-]+)\,//)
{
my $segment_start=$1;
if ($segment_lengths_=~s/^([0-9]+)\,//)
{
my $segment_length=$1;
$seqment_lengths_sum+=$segment_length;
#print qq!$name $seqment_count. $seqment_lengths_sum\n!;
my $seq_=substr $sequence,$start+$segment_start,$segment_length;
my $seq__="";
my $changed=0;
for(my $i=0,my $j=$start+$segment_start;$i<$segment_length;$i++,$j++)
{
my $temp="";
if ($vcf_new{"$chr#$j"}=~/\w/ and $vcf_new{"$chr#$j"}!~/^$vcf_old{"$chr#$j"}$/ and length($vcf_new{"$chr#$j"})==length($vcf_old{"$chr#$j"}))
{
my $n=substr $seq_,$i,length($vcf_old{"$chr#$j"});
print qq!$chr#$j: $n $vcf_new{"$chr#$j"}\n!;
if ($n!~/^$vcf_old{"$chr#$j"}$/)
{
$temp=qq! (Warning: $n\!\=$vcf_old{"$chr#$j"}) - Ignored!;
my $i_=$i+$segment_start_first+length($seq);
# print LOG_MOD qq!Variant $chr $j: $n,$vcf_old{"$chr#$j"}$temp->$vcf_new{"$chr#$j"} $i_\n!;
$count_variant_in_exon_old_error++;
if ($vcf_type{"$chr#$j"}=~/S/)
{
$count_variant_in_exon_old_error_somatic++;
}
}
else
{
my $i_=$i+$segment_start_first+length($seq);
if ($i_>=0)
{
$modified++;
$seq__.=$vcf_new{"$chr#$j"};
$changed=length($vcf_old{"$chr#$j"});
# print LOG_MOD qq!Variant $chr $j: $vcf_type{"$chr#$j"} $n,$vcf_old{"$chr#$j"}->$vcf_new{"$chr#$j"} $i_\n!;
#$description_.=qq!$chr-$j($i_)-$vcf_type{"$chr#$j"}:$vcf_old{"$chr#$j"}->$vcf_new{"$chr#$j"}, !;
$count_variant_in_exon++;
if ($vcf_type{"$chr#$j"}=~/S/)
{
$count_variant_in_exon_somatic++;
}
$variants{$i_}=qq!$vcf_old{"$chr#$j"}#$vcf_new{"$chr#$j"}#$vcf_type{"$chr#$j"}!;
$variants2{$i_}=qq!$chr-$j!;
# print LOG_MOD qq!-$i_. $variants{$i_} $variants2{$i_}\n!;
}
else
{
print LOG_MOD qq!Ignored: Variant $chr $j: $vcf_type{"$chr#$j"} $n,$vcf_old{"$chr#$j"}->$vcf_new{"$chr#$j"} $i_\n!;
}
}
}
if ($changed<=0) { $seq__.=substr $seq_,$i,1; $changed=0; } else { $changed--; }
}
# print LOG_MOD qq!$start+$segment_start,$segment_length: $seq__\n!;
$seq.=$seq__;
$seqment_count++;
} else { print LOG_MOD qq!Error parsing $bed{$name}\n!; }
}
my $name_=$name; $name_=~s/\-[^\-]+$//;
#my $gene=$name; $gene=~s/^[^\-]+\-//;
my $protein="";
my $protein_original="";
if ($strand=~/\-/)
{
my $seq_ = reverse $seq;
$seq=$seq_;
$seq=~tr/ATCG/TAGC/;
$seq_ = reverse $seq_original;
$seq_original=$seq_;
$seq_original=~tr/ATCG/TAGC/;
foreach my $key (keys %variants)
{
my $key_=length($seq)+$segment_start_first-$key-1;
$variants_{$key_}=$variants{$key};
$variants2_{$key_}=$variants2{$key};
#print qq!$key->$key_\n!;
}
}
else
{
foreach my $key (keys %variants)
{
$variants_{$key}=$variants{$key};
$variants2_{$key}=$variants2{$key};
}
}
for(my $n=0;$n<length($seq_original);$n=$n+3)
{
my $triplet = substr($seq_original, $n, 3);
if (length($triplet)==3)
{
if ($mapping{$triplet}!~/[\w\*]/) { $mapping{$triplet}="X"; }
$protein_original.=$mapping{$triplet};
}
}
$modified=0;
my $modified_somatic=0;
my $stop_found=0;
my $triplet_count=0;
my $frame_shift=0;
#if ($description_=~/\w/) { $description_.="; "; }
for(my $n=0;$n<length($seq) and $stop_found==0;$n=$n+3)
{
my $n_=$n+2;
my $triplet_count_=$triplet_count+1;
my $triplet = substr($seq, $n, 3);
if (length($triplet)==3)
{
if ($mapping{$triplet}!~/[\w\*]/) { $mapping{$triplet}="X"; }
my $triplet_old=$triplet;
my $triplet_type="";
my $frame_shift_this=0;
for (my $i=$n, my $j=0; $i<=$n_; $i++,$j++)
{
#print LOG_MOD qq!$i. $variants_{$i}\n!;
if ($variants_{$i}=~/^([^#]+)#([^#]+)#([^#]+)$/)
{
my $old=$1;
my $new=$2;
$frame_shift += abs(length($old)-length($new)) % 3;
$frame_shift_this = abs(length($old)-length($new)) % 3;
$triplet_type=$3;
if ($strand=~/\-/) { $old=~tr/ATCG/TAGC/; }
substr $triplet_old,$j,1,$old;
$variant_loc=$variants2_{$i};
#print LOG_MOD qq!$i. $triplet $triplet_old $old $variant_loc $variants2_{$i}\n!;
}
}
if ($mapping{$triplet_old}!~/[\w\*]/) { $mapping{$triplet_old}="X"; }
#print LOG_MOD qq!$name $triplet_type $n-$n_: $triplet_old->$triplet - $triplet_count_:$mapping{$triplet_old}->$mapping{$triplet}\n!;
if ($mapping{$triplet}=~/\*/)
{
$stop_found=1;
}
if ($mapping{$triplet}=~/\*/ and $mapping{$triplet_old}!~/\*/ and $frame_shift==0)
{
$protein.=$mapping{$triplet_old};
# print LOG_MOD qq!$triplet_type $n-$n_:$triplet_old->$triplet - $triplet_count_:$mapping{$triplet_old}->$mapping{$triplet}\n!;
#$description_.=qq!$variant_loc-$triplet_type:$mapping{$triplet_old}$triplet_count_$mapping{$triplet},!;
$modified++;
$count_variant_in_exon_nonsyn++;
$count_stop_introduced++;
if ($triplet_type=~/S/)
{
$modified_somatic++;
$count_variant_in_exon_nonsyn_somatic++;
$count_stop_introduced_somatic++;
}
}
else
{
if ($mapping{$triplet}!~/\*/ and $mapping{$triplet_old}=~/\*/ and $frame_shift==0)
{
$protein.=$mapping{$triplet};
# print LOG_MOD qq!$triplet_type $n-$n_:$triplet_old->$triplet - $triplet_count_:$mapping{$triplet_old}->$mapping{$triplet}\n!;
#$description_.=qq!$variant_loc-$triplet_type:$mapping{$triplet_old}$triplet_count_$mapping{$triplet},!;
$modified++;
$count_variant_in_exon_nonsyn++;
$count_stop_removed++;
if ($triplet_type=~/S/)
{
$modified_somatic++;
$count_variant_in_exon_nonsyn_somatic++;
$count_stop_removed_somatic++;
}
}
else
{
if ($mapping{$triplet}!~/^$mapping{$triplet_old}$/ and $frame_shift==0)
{
$protein.=$mapping{$triplet};
# print LOG_MOD qq!$triplet_type $n-$n_:$triplet_old->$triplet - $triplet_count_:$mapping{$triplet_old}->$mapping{$triplet}\n!;
#$description_.=qq!$variant_loc-$triplet_type $n-$n_:$triplet_old->$triplet - $triplet_count_:$mapping{$triplet_old}->$mapping{$triplet},!;
$description_.=qq!$variant_loc-$triplet_type:$mapping{$triplet_old}$triplet_count_$mapping{$triplet},!;
$modified++;
$count_variant_in_exon_nonsyn++;
if ($triplet_type=~/S/)
{
$modified_somatic++;
$count_variant_in_exon_nonsyn_somatic++;
}
}
else
{
if ($frame_shift_this!=0)
{
$protein.=$mapping{$triplet_old};
$frame_shift_this=0;
# print LOG_MOD qq!$triplet_type $n-$n_:$triplet_old->$triplet - $triplet_count_:$mapping{$triplet_old}->$mapping{$triplet}\n!;
#$description_.=qq!$variant_loc-$triplet_type:$triplet_count_-frame-shift,!;
$modified++;
$count_variant_in_exon_nonsyn++;
if ($triplet_type=~/S/)
{
$modified_somatic++;
$count_variant_in_exon_nonsyn_somatic++;
}
}
else
{
$protein.=$mapping{$triplet_old};
}
}
}
}
}
$triplet_count++;
}
$proteins_count++;
$protein_modifications_count+=$modified;
$protein_modifications_distr[$modified]++;
$protein_modifications_count_somatic+=$modified_somatic;
$protein_modifications_distr_somatic[$modified_somatic]++;
$protein_original=~s/\*$//;
if ($protein_original=~/^([^\*]+)\*.*$/)
{
print LOG qq!Error: Stop codon found in middle of sequence:$name \n$protein_original\n!;
$protein_original="";
}
# if (length($protein_original)>6 and $protein_original!~/^\*/)
# {
# print OUT qq!>$name $descriptions{$name} (MAP:$chr:$start$strand $segment_lengths $segment_starts)\n$protein_original\n!;
# }
if ($modified!=0)
{
$protein=~s/\*$//;
$protein=~s/^([^\*]+)\*.*$//;
$proteins_modified++;
if (length($protein)>6 and $protein!~/^\*/)
{
$description_.=")";
# print LOG_MOD qq!>$name-variant $descriptions{$name} (MAP:$chr:$start$strand $segment_lengths $segment_starts) $description_\n$protein\n!;
print OUT_MOD qq!>$name-variant $descriptions{$name} (MAP:$chr:$start$strand $segment_lengths $segment_starts) $description_\n$protein\n!;
}
if (length($protein_original)>6 and $protein_original!~/^\*/)
{
print OUT qq!>$name $descriptions{$name} (MAP:$chr:$start$strand $segment_lengths $segment_starts)\n$protein_original\n!;
}
}
} else { print LOG qq!Error parsing $bed{$name}\n!; }
}
} else { print LOG qq!Error in name $chr: $line\n!; }
close(IN);
}
}
print STAT qq!
$proteins_count proteins
$proteins_modified proteins modified
$count_stop_removed stop codons removed
$count_stop_introduced stop codons introduced
Somatic: $count_stop_removed_somatic stop codons removed
Somatic: $count_stop_introduced_somatic stop codons introduced
$protein_modifications_count total modifications
$protein_modifications_count_somatic somatic modifications
Somatic: $somatic_only
Germline: $germline_only
In both VCF: $both_vcf (differ: $both_vcf_differ)
$count_variant_in_exon variants in exons
$count_variant_in_exon_old_error variants in exons where old does not match genome
$count_variant_in_exon_nonsyn non-synonymous variants
Somatic: $count_variant_in_exon_somatic variants in exons
Somatic: $count_variant_in_exon_old_error_somatic variants in exons where old does not match genome
Somatic: $count_variant_in_exon_nonsyn_somatic non-synonymous variants
!;
print STAT qq!\nnumber of modifications\tnumber of proteins\tnumber of proteins (somatic variants)\n!;
#for (my $k=0;$k<=100;$k++) { print STAT qq!$k\t$protein_modifications_distr[$k]\t$protein_modifications_distr_somatic[$k]\n!; }
close(OUT);
close(OUT_MOD);
close(OUT_FS);
close(LOG);
close(LOG_MOD);
close(STAT);
}