diff --git a/core/src/components/segment-button/segment-button.tsx b/core/src/components/segment-button/segment-button.tsx
index d860735f729..3a2135421c2 100644
--- a/core/src/components/segment-button/segment-button.tsx
+++ b/core/src/components/segment-button/segment-button.tsx
@@ -2,7 +2,7 @@ import type { ComponentInterface } from '@stencil/core';
 import { Component, Element, Host, Prop, Method, State, Watch, forceUpdate, h } from '@stencil/core';
 import type { ButtonInterface } from '@utils/element-interface';
 import type { Attributes } from '@utils/helpers';
-import { addEventListener, removeEventListener, inheritAttributes } from '@utils/helpers';
+import { addEventListener, removeEventListener, inheritAttributes, getNextSiblingOfType } from '@utils/helpers';
 import { hostContext } from '@utils/theme';
 
 import { getIonMode } from '../../global/ionic-global';
@@ -65,7 +65,41 @@ export class SegmentButton implements ComponentInterface, ButtonInterface {
     this.updateState();
   }
 
-  connectedCallback() {
+  private waitForSegmentContent(ionSegment: HTMLIonSegmentElement | null, contentId: string): Promise<HTMLElement> {
+    return new Promise((resolve, reject) => {
+      let timeoutId: NodeJS.Timeout | undefined = undefined;
+      let animationFrameId: number;
+
+      const check = () => {
+        if (!ionSegment) {
+          reject(new Error(`Segment not found when looking for Segment Content`));
+          return;
+        }
+
+        const segmentView = getNextSiblingOfType<HTMLIonSegmentViewElement>(ionSegment); // Skip the text nodes
+        const segmentContent = segmentView?.querySelector(
+          `ion-segment-content[id="${contentId}"]`
+        ) as HTMLIonSegmentContentElement | null;
+        if (segmentContent && timeoutId) {
+          clearTimeout(timeoutId); // Clear the timeout if the segmentContent is found
+          cancelAnimationFrame(animationFrameId);
+          resolve(segmentContent);
+        } else {
+          animationFrameId = requestAnimationFrame(check); // Keep checking on the next animation frame
+        }
+      };
+
+      check();
+
+      // Set a timeout to reject the promise
+      timeoutId = setTimeout(() => {
+        cancelAnimationFrame(animationFrameId);
+        reject(new Error(`Unable to find Segment Content with id="${contentId} within 1000 ms`));
+      }, 1000);
+    });
+  }
+
+  async connectedCallback() {
     const segmentEl = (this.segmentEl = this.el.closest('ion-segment'));
     if (segmentEl) {
       this.updateState();
@@ -76,12 +110,13 @@ export class SegmentButton implements ComponentInterface, ButtonInterface {
     // Return if there is no contentId defined
     if (!this.contentId) return;
 
-    // Attempt to find the Segment Content by its contentId
-    const segmentContent = document.getElementById(this.contentId) as HTMLIonSegmentContentElement | null;
-
-    // If no associated Segment Content exists, log an error and return
-    if (!segmentContent) {
-      console.error(`Segment Button: Unable to find Segment Content with id="${this.contentId}".`);
+    let segmentContent;
+    try {
+      // Attempt to find the Segment Content by its contentId
+      segmentContent = await this.waitForSegmentContent(segmentEl, this.contentId);
+    } catch (error) {
+      // If no associated Segment Content exists, log an error and return
+      console.error('Segment Button: ', (error as Error).message);
       return;
     }
 
diff --git a/core/src/components/segment-view/test/basic/index.html b/core/src/components/segment-view/test/basic/index.html
index 69d36d4a6c0..78dec1d9ff9 100644
--- a/core/src/components/segment-view/test/basic/index.html
+++ b/core/src/components/segment-view/test/basic/index.html
@@ -123,6 +123,8 @@
         <button class="expand" onClick="changeSegmentContent()">Change Segment Content</button>
 
         <button class="expand" onClick="clearSegmentValue()">Clear Segment Value</button>
+
+        <button class="expand" onClick="addSegmentButtonAndContent()">Add New Segment Button & Content</button>
       </ion-content>
 
       <ion-footer>
@@ -158,6 +160,34 @@
             segment.value = undefined;
           });
         }
+
+        async function addSegmentButtonAndContent() {
+          const segment = document.querySelector('ion-segment');
+          const segmentView = document.querySelector('ion-segment-view');
+
+          const newButton = document.createElement('ion-segment-button');
+          const newId = `new-${Date.now()}`;
+          newButton.setAttribute('content-id', newId);
+          newButton.setAttribute('value', newId);
+          newButton.innerHTML = '<ion-label>New Button</ion-label>';
+
+          segment.appendChild(newButton);
+
+          setTimeout(() => {
+            // Timeout to test waitForSegmentContent() in segment-button
+            const newContent = document.createElement('ion-segment-content');
+            newContent.setAttribute('id', newId);
+            newContent.innerHTML = 'New Content';
+
+            segmentView.appendChild(newContent);
+
+            // Necessary timeout to ensure the value is set after the content is added.
+            // Otherwise, the transition is unsuccessful and the content is not shown.
+            setTimeout(() => {
+              segment.setAttribute('value', newId);
+            }, 200);
+          }, 200);
+        }
       </script>
     </ion-app>
   </body>
diff --git a/core/src/utils/helpers.ts b/core/src/utils/helpers.ts
index 97681f6652a..a740ff98ac7 100644
--- a/core/src/utils/helpers.ts
+++ b/core/src/utils/helpers.ts
@@ -413,3 +413,14 @@ export const shallowEqualStringMap = (
 
   return true;
 };
+
+export const getNextSiblingOfType = <T extends Element>(element: Element): T | null => {
+  let sibling = element.nextSibling;
+  while (sibling) {
+    if (sibling.nodeType === Node.ELEMENT_NODE && (sibling as T) !== null) {
+      return sibling as T;
+    }
+    sibling = sibling.nextSibling;
+  }
+  return null;
+};