diff --git a/config/jobs/kubernetes/sig-node/sig-node-presubmit.yaml b/config/jobs/kubernetes/sig-node/sig-node-presubmit.yaml
index 8efe04a927b1..e4ac52b60528 100644
--- a/config/jobs/kubernetes/sig-node/sig-node-presubmit.yaml
+++ b/config/jobs/kubernetes/sig-node/sig-node-presubmit.yaml
@@ -1708,6 +1708,63 @@ presubmits:
         env:
         - name: IGNITION_INJECT_GCE_SSH_PUBLIC_KEY_FILE
           value: "1"
+  - name: pull-kubernetes-node-kubelet-serial-crio-cgroupv1-kubetest2
+    cluster: k8s-infra-prow-build
+    # explicitly needs /test pull-kubernetes-node-kubelet-serial-crio-cgroupv1-kubetest2 to run
+    always_run: false
+    optional: true
+    skip_report: false
+    max_concurrency: 12
+    skip_branches:
+    - release-\d+\.\d+  # per-release image
+    decorate: true
+    path_alias: k8s.io/kubernetes
+    extra_refs:
+    - org: kubernetes
+      repo: test-infra
+      base_ref: master
+      path_alias: k8s.io/test-infra
+    decoration_config:
+      timeout: 4h
+    labels:
+      preset-service-account: "true"
+      preset-k8s-ssh: "true"
+      preset-pull-kubernetes-e2e: "true"
+      preset-pull-kubernetes-e2e-gce: "true"
+    annotations:
+      testgrid-dashboards: sig-node-cri-o, sig-node-presubmits
+      testgrid-tab-name: pr-node-kubelet-serial-crio-cgroupv1-kubetest2
+    spec:
+      containers:
+      - image: gcr.io/k8s-staging-test-infra/kubekins-e2e:v20240923-c8645c1a17-master
+        command:
+        - runner.sh
+        args:
+        - kubetest2
+        - noop
+        - --test=node
+        - --
+        - --repo-root=.
+        - --gcp-zone=us-west1-b
+        - --parallelism=1
+        - --focus-regex=\[Serial\]
+        - --skip-regex=\[Flaky\]|\[Benchmark\]|\[NodeSpecialFeature:.+\]|\[NodeSpecialFeature\]|\[NodeAlphaFeature:.+\]|\[NodeAlphaFeature\]|\[NodeFeature:Eviction\]|\[Feature:CPUManager\]|\[Feature:MemoryManager\]|\[Feature:TopologyManager\]
+        - '--test-args=--container-runtime-endpoint=unix:///var/run/crio/crio.sock --container-runtime-process-name=/usr/local/bin/crio --container-runtime-pid-file= --kubelet-flags="--cgroup-driver=systemd --cgroups-per-qos=true --cgroup-root=/ --runtime-cgroups=/system.slice/crio.service --kubelet-cgroups=/system.slice/kubelet.service" --extra-log="{\"name\": \"crio.log\", \"journalctl\": [\"-u\", \"crio\"]}"'
+        - --image-config-file=/home/prow/go/src/k8s.io/test-infra/jobs/e2e_node/crio/latest/image-config-cgroupv1-serial.yaml
+        resources:
+          limits:
+            cpu: 4
+            memory: 12Gi
+          requests:
+            cpu: 4
+            memory: 12Gi
+        env:
+          - name: KUBE_SSH_KEY_PATH
+            value: /etc/ssh-key-secret/ssh-private
+          - name: KUBE_SSH_USER
+            value: core
+          - name: IGNITION_INJECT_GCE_SSH_PUBLIC_KEY_FILE
+            value: "1"
   - name: pull-kubernetes-node-kubelet-serial-crio-cgroupv2
     cluster: k8s-infra-prow-build
     skip_branches: