From f185c2af6904be02a4a822bace364ad162bca0c0 Mon Sep 17 00:00:00 2001 From: Lukasz Jablonski Date: Tue, 12 Jul 2016 12:27:26 +0200 Subject: [PATCH] First release --- bibliography.bib | 40 ++++ publication_list.bib | 27 +++ thesis.lyx | 536 +++++++++++++++++++++++++++++++++++++++++++ thesis.pdf | Bin 0 -> 108719 bytes 4 files changed, 603 insertions(+) create mode 100644 bibliography.bib create mode 100644 publication_list.bib create mode 100644 thesis.lyx create mode 100644 thesis.pdf diff --git a/bibliography.bib b/bibliography.bib new file mode 100644 index 0000000..bb0c6ea --- /dev/null +++ b/bibliography.bib @@ -0,0 +1,40 @@ +%% This BibTeX bibliography file was created using BibDesk. +%% http://bibdesk.sourceforge.net/ + + +%% Created for Lukasz Jablonski at 2016-07-12 12:25:26 +0200 + + +%% Saved with string encoding Unicode (UTF-8) + + + +@article{Delvendahl:2015aa, + Abstract = {Fast synchronous neurotransmitter release at the presynaptic active zone is triggered by local Ca2+ signals, which are confined in their spatiotemporal extent by endogenous Ca2+ buffers. However, it remains elusive how rapid and reliable Ca2+ signaling can be sustained during repetitive release. Here, we established quantitative two-photon Ca2+ imaging in cerebellar mossy fiber boutons, which fire at exceptionally high rates. We show that endogenous fixed buffers have a surprisingly low Ca2+-binding ratio (∼15) and low affinity, whereas mobile buffers have high affinity. Experimentally constrained modeling revealed that the low endogenous buffering promotes fast clearance of Ca2+ from the active zone during repetitive firing. Measuring Ca2+ signals at different distances from active zones with ultra-high-resolution confirmed our model predictions. Our results lead to the concept that reduced Ca2+ buffering enables fast active zone Ca2+ signaling, suggesting that the strength of endogenous Ca2+ buffering limits the rate of synchronous synaptic transmission.}, + Author = {Delvendahl, Igor and Jablonski, Lukasz and Baade, Carolin and Matveev, Victor and Neher, Erwin and Hallermann, Stefan}, + Date-Added = {2016-02-04 10:04:09 +0000}, + Date-Modified = {2016-07-12 10:24:22 +0000}, + Eprint = {http://www.pnas.org/content/112/23/E3075.full.pdf}, + Journal = {Proceedings of the National Academy of Sciences}, + Number = {23}, + Pages = {E3075-E3084}, + Title = {Reduced endogenous {Ca$^{2+}$} buffering speeds active zone {Ca$^{2+}$} signaling}, + Volume = {112}, + Year = {2015}, + Bdsk-Url-1 = {http://www.pnas.org/content/112/23/E3075.abstract}, + Bdsk-Url-2 = {http://dx.doi.org/10.1073/pnas.1508419112}} + +@comment{BibDesk Static Groups{ + + + + + + group name + NOT USED? + keys + + + + +}} diff --git a/publication_list.bib b/publication_list.bib new file mode 100644 index 0000000..e646a37 --- /dev/null +++ b/publication_list.bib @@ -0,0 +1,27 @@ +%% This BibTeX bibliography file was created using BibDesk. +%% http://bibdesk.sourceforge.net/ + + +%% Created for Lukasz Jablonski at 2016-02-04 12:08:40 +0100 + + +%% Saved with string encoding Unicode (UTF-8) + + + +@article{Delvendahl:2015aa, + Abstract = {Fast synchronous neurotransmitter release at the presynaptic active zone is triggered by local Ca2+ signals, which are confined in their spatiotemporal extent by endogenous Ca2+ buffers. However, it remains elusive how rapid and reliable Ca2+ signaling can be sustained during repetitive release. Here, we established quantitative two-photon Ca2+ imaging in cerebellar mossy fiber boutons, which fire at exceptionally high rates. We show that endogenous fixed buffers have a surprisingly low Ca2+-binding ratio (∼15) and low affinity, whereas mobile buffers have high affinity. Experimentally constrained modeling revealed that the low endogenous buffering promotes fast clearance of Ca2+ from the active zone during repetitive firing. Measuring Ca2+ signals at different distances from active zones with ultra-high-resolution confirmed our model predictions. Our results lead to the concept that reduced Ca2+ buffering enables fast active zone Ca2+ signaling, suggesting that the strength of endogenous Ca2+ buffering limits the rate of synchronous synaptic transmission.}, + Author = {Delvendahl, Igor and Jablonski, Lukasz and Baade, Carolin and Matveev, Victor and Neher, Erwin and Hallermann, Stefan}, + Date-Added = {2016-02-04 10:04:09 +0000}, + Date-Modified = {2016-02-04 10:37:29 +0000}, + Doi = {10.1073/pnas.1508419112}, + Eprint = {http://www.pnas.org/content/112/23/E3075.full.pdf}, + Journal = {Proceedings of the National Academy of Sciences}, + Number = {23}, + Pages = {E3075-E3084}, + Title = {Reduced endogenous {Ca$^{2+}$} buffering speeds active zone {Ca$^{2+}$} signaling}, + Url = {http://www.pnas.org/content/112/23/E3075.abstract}, + Volume = {112}, + Year = {2015}, + Bdsk-Url-1 = {http://www.pnas.org/content/112/23/E3075.abstract}, + Bdsk-Url-2 = {http://dx.doi.org/10.1073/pnas.1508419112}} diff --git a/thesis.lyx b/thesis.lyx new file mode 100644 index 0000000..9d2544b --- /dev/null +++ b/thesis.lyx @@ -0,0 +1,536 @@ +#LyX 2.2 created this file. 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