diff --git a/.github/workflows/pyinstaller_windows.yml b/.github/workflows/pyinstaller_windows.yml
new file mode 100644
index 00000000000..cd6f89c1a5c
--- /dev/null
+++ b/.github/workflows/pyinstaller_windows.yml
@@ -0,0 +1,81 @@
+name: PyInstaller Windows Build
+
+on:
+  pull_request:
+    branches:
+      - 'main'
+      - 'release-*'
+  release:
+
+jobs:
+  test:
+    runs-on: windows-latest
+
+    steps:
+      - uses: actions/checkout@v3
+        with:
+          fetch-depth: 0
+
+      - name: Get Date
+        id: get-date
+        run: |
+          echo "::set-output name=date::$(/bin/date -u "+%Y%V")"
+        shell: bash
+
+      - name: Cache Mambaforge and Pip packages
+        uses: actions/cache@v2
+        env:
+          CACHE_NUMBER: 0
+        with:
+          path: |
+            ~/conda_pkgs_dir
+            ~/.cache/pip
+          key:
+            ${{runner.os}}-condapkg-${{env.CACHE_NUMBER}}-${{steps.get-date.outputs.date}}-${{hashFiles('environment-dev.yml','conda/meta.yaml')}}
+
+      - name: Setup Mambaforge
+        uses: conda-incubator/setup-miniconda@v2.1.1
+        with:
+          miniforge-version: latest
+          miniforge-variant: Mambaforge
+          activate-environment: mantidimaging-dev
+          auto-activate-base: false
+          use-mamba: true
+
+      - name: Cache Mambaforge environment
+        uses: actions/cache@v2
+        id: cache-mambaforge-environment
+        env:
+          CACHE_NUMBER: 0
+        with:
+          path: ${{env.CONDA}}/envs/mantidimaging-dev
+          key:
+            ${{runner.os}}-condaenv-${{env.CACHE_NUMBER}}-${{steps.get-date.outputs.date}}-${{hashFiles('environment-dev.yml','conda/meta.yaml')}}
+
+      - name: Mantid Imaging developer dependencies
+        if: steps.cache-mambaforge-environment.outputs.cache-hit != 'true'
+        shell: bash -l {0}
+        run: |
+          conda deactivate
+          python ./setup.py create_dev_env
+          
+      - name: cuda-toolkit
+        uses: Jimver/cuda-toolkit@v0.2.8
+        with:
+          cuda: '10.2.89'
+
+      - name: PyInstaller Build
+        shell: bash -l {0}
+        run: |
+          conda activate mantidimaging-dev
+          mamba install -y cupy=10.2.* --force-reinstall
+          echo "Cuda path: $CUDA_PATH"
+          pip install pyinstaller
+          cd packaging
+          python PackageWithPyInstaller.py
+
+      - name: Archive PyInstalled File
+        uses: actions/upload-artifact@v3
+        with:
+          name: MantidImaging
+          path: packaging/dist
diff --git a/docs/release_notes/next.rst b/docs/release_notes/next.rst
index bf4ae3834f8..47ead424b15 100644
--- a/docs/release_notes/next.rst
+++ b/docs/release_notes/next.rst
@@ -47,3 +47,4 @@ Developer Changes
 - #1673 : Update license year across the repository from 2022 to 2023
 - #1678 : Allow multiple datasets to be opened at once in the GUI when using the CLI `--path` flag
 - #1695 : Use FilenameGroup for Load Images
+- #1683 : Add single-file PyInstaller build to Actions
diff --git a/packaging/PackageWithPyInstaller.py b/packaging/PackageWithPyInstaller.py
index 4d9aae970dc..114c3e43004 100644
--- a/packaging/PackageWithPyInstaller.py
+++ b/packaging/PackageWithPyInstaller.py
@@ -14,7 +14,7 @@
 
 def create_run_options():
     run_options = [
-        '../mantidimaging/__main__.py', '--name=MantidImaging', '--additional-hooks-dir=hooks', '--onefile',
+        '../mantidimaging/__main__.py', '--name=MantidImaging', '--additional-hooks-dir=hooks',
         '--icon=../images/mantid_imaging_unstable_64px.ico'
     ]