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Bowtie2 error #19

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mredrejo opened this issue Feb 15, 2021 · 0 comments
Open

Bowtie2 error #19

mredrejo opened this issue Feb 15, 2021 · 0 comments

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@mredrejo
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I got the following Bowtie2 error:

Error in rule bowtie2_map:
jobid: 8
output: ctrl_mc/assembly/mc.index, ctrl_mc/assembly/mc.index, ctrl_mc/assembly/mc.sam, ctrl_mc/logs/bowtie2map.log
shell:
bowtie2-build -o 3 --threads 18 -q ctrl_mc/reference_selection/mc.refseq.fna ctrl_mc/assembly/mc.index 1>> ctrl_mc/assembly/mc.index 2>&1;bowtie2 -a --end-to-end --sensitive --no-unal -p 18 -x ctrl_mc/assembly/mc.index -q -U ctrl_R1_paired.fastq,ctrl_R2_paired.fastq,ctrl_unpaired.fastq -S ctrl_mc/assembly/mc.sam.all > ctrl_mc/logs/bowtie2map.log 2>&1; python3 /home/modesto/Applications/MetaCompass-2.0-beta/bin/best_strata.py ctrl_mc/assembly/mc.sam.all ctrl_mc/assembly/mc.sam; rm ctrl_mc/assembly/mc.sam.all && touch ctrl_mc/assembly/.run1.ok
(one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)

I just updated all dependencies before using. I tried the 1.12 and 2.0Beta releases and I got the same error. Running on Ubuntu 20.04.1 LTS.

Thanks

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