diff --git a/bson/binary.py b/bson/binary.py
index a1f63adf27..926263eee7 100644
--- a/bson/binary.py
+++ b/bson/binary.py
@@ -14,6 +14,7 @@
 from __future__ import annotations
 
 import struct
+import warnings
 from enum import Enum
 from typing import TYPE_CHECKING, Any, Optional, Sequence, Tuple, Type, Union, overload
 from uuid import UUID
@@ -255,6 +256,9 @@ def __eq__(self, other: Any) -> bool:
             self.dtype == other.dtype and self.padding == other.padding and self.data == other.data
         )
 
+    def __len__(self) -> int:
+        return len(self.data)
+
 
 class Binary(bytes):
     """Representation of BSON binary data.
@@ -471,6 +475,10 @@ def from_vector(
 
         metadata = struct.pack("<sB", dtype.value, padding)
         data = struct.pack(f"<{len(vector)}{format_str}", *vector)  # type: ignore
+        if padding and len(vector) and not (data[-1] & ((1 << padding) - 1)) == 0:
+            raise ValueError(
+                "Vector has a padding P, but bits in the final byte lower than P are non-zero. They must be zero."
+            )
         return cls(metadata + data, subtype=VECTOR_SUBTYPE)
 
     def as_vector(self) -> BinaryVector:
@@ -522,6 +530,12 @@ def as_vector(self) -> BinaryVector:
             dtype_format = "B"
             format_string = f"<{n_values}{dtype_format}"
             unpacked_uint8s = list(struct.unpack_from(format_string, self, position))
+            if padding and n_values and unpacked_uint8s[-1] & (1 << padding) - 1 != 0:
+                warnings.warn(
+                    "Vector has a padding P, but bits in the final byte lower than P are non-zero. For pymongo>=5.0, they must be zero.",
+                    DeprecationWarning,
+                    stacklevel=2,
+                )
             return BinaryVector(unpacked_uint8s, dtype, padding)
 
         else:
diff --git a/doc/changelog.rst b/doc/changelog.rst
index ca4784f919..6363cd7bcb 100644
--- a/doc/changelog.rst
+++ b/doc/changelog.rst
@@ -51,6 +51,9 @@ PyMongo 4.13 brings a number of changes including:
   or the `migration guide <https://www.mongodb.com/docs/languages/python/pymongo-driver/current/reference/migration/>`_ for more information.
 - Fixed a bug where :class:`pymongo.write_concern.WriteConcern` repr was not eval-able
   when using ``w="majority"``.
+- Ignored bits in a BSON BinaryVector of PACKED_BIT dtype should be set to zero.
+  On writes, this is enforced and is a breaking change.
+  Reads from the database will not fail, however a warning will be triggered.
 
 Issues Resolved
 ...............
diff --git a/test/test_bson.py b/test/test_bson.py
index 23e0a29c4f..4d25d67ebe 100644
--- a/test/test_bson.py
+++ b/test/test_bson.py
@@ -739,7 +739,7 @@ def test_vector(self):
         """Tests of subtype 9"""
         # We start with valid cases, across the 3 dtypes implemented.
         # Work with a simple vector that can be interpreted as int8, float32, or ubyte
-        list_vector = [127, 7]
+        list_vector = [127, 8]
         # As INT8, vector has length 2
         binary_vector = Binary.from_vector(list_vector, BinaryVectorDtype.INT8)
         vector = binary_vector.as_vector()
@@ -764,18 +764,18 @@ def test_vector(self):
         uncompressed = ""
         for val in list_vector:
             uncompressed += format(val, "08b")
-        assert uncompressed[:-padding] == "0111111100000"
+        assert uncompressed[:-padding] == "0111111100001"
 
         # It is worthwhile explicitly showing the values encoded to BSON
         padded_doc = {"padded_vec": padded_vec}
         assert (
             encode(padded_doc)
-            == b"\x1a\x00\x00\x00\x05padded_vec\x00\x04\x00\x00\x00\t\x10\x03\x7f\x07\x00"
+            == b"\x1a\x00\x00\x00\x05padded_vec\x00\x04\x00\x00\x00\t\x10\x03\x7f\x08\x00"
         )
         # and dumped to json
         assert (
             json_util.dumps(padded_doc)
-            == '{"padded_vec": {"$binary": {"base64": "EAN/Bw==", "subType": "09"}}}'
+            == '{"padded_vec": {"$binary": {"base64": "EAN/CA==", "subType": "09"}}}'
         )
 
         # FLOAT32 is also implemented
@@ -784,15 +784,14 @@ def test_vector(self):
 
         # Now some invalid cases
         for x in [-1, 257]:
-            try:
+            with self.assertRaises(struct.error):
                 Binary.from_vector([x], BinaryVectorDtype.PACKED_BIT)
-            except Exception as exc:
-                self.assertIsInstance(exc, struct.error)
-            else:
-                self.fail("Failed to raise an exception.")
 
-        # Test form of Binary.from_vector(BinaryVector)
+        # Test one must pass zeros for all ignored bits
+        with self.assertRaises(ValueError):
+            Binary.from_vector([255], BinaryVectorDtype.PACKED_BIT, padding=7)
 
+        # Test form of Binary.from_vector(BinaryVector)
         assert padded_vec == Binary.from_vector(
             BinaryVector(list_vector, BinaryVectorDtype.PACKED_BIT, padding)
         )