diff --git a/README.md b/README.md index eaa8b03..fdbb3c4 100644 --- a/README.md +++ b/README.md @@ -88,7 +88,7 @@ Each step of the installation process is expected to take a matter of seconds. | Command | Default | Description | | :------- | :----- | :-------- | -|--type | map-ont | denote sequencer [short-read:sr, Pac-Bio:map-pb, ONT:map-ont] | +|--type | map-ont | denote sequencer [short-read:sr, Pac-Bio:map-pb, ONT:map-ont, Nanopore Q20:lr:hq, PacBio HiFi:map-hifi, traditional cDNA:splice:hq] | |--min-abundance| 0.0001| generates results with species relative abundance above this value in addition to full results; .01 = 1%| |--db| $EMU_DATABASE_DIR| path to emu database; directory must include the following files: names_df.tsv, nodes_df.tsv, species_taxid.fasta, unqiue_taxids.tsv| |--N| 50| max number of alignments utilized for each read in minimap2| diff --git a/emu b/emu index e60b751..4df92c5 100755 --- a/emu +++ b/emu @@ -697,8 +697,8 @@ if __name__ == "__main__": 'input_file', type=str, nargs='+', help='filepath to input nt sequence file') abundance_parser.add_argument( - '--type', '-x', choices=['map-ont', 'map-pb', 'sr'], default='map-ont', - help='short-read: sr, Pac-Bio:map-pb, ONT:map-ont [map-ont]') + '--type', '-x', choices=['map-ont', 'map-pb', 'sr', 'lr:hq', 'map-hifi', 'splice:hq'], default='map-ont', + help='short-read: sr, Pac-Bio:map-pb, ONT:map-ont, ... [map-ont]') abundance_parser.add_argument( '--min-abundance', '-a', type=float, default=0.0001, help='min species abundance in results [0.0001]') diff --git a/environment.yml b/environment.yml index aa5fe9f..3e7bdb6 100644 --- a/environment.yml +++ b/environment.yml @@ -11,4 +11,4 @@ dependencies: - numpy >=1.11 - pandas >=1.1 - flatten-dict - - minimap2 >=2.22 + - minimap2 >=2.27