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New issue of Markerminer with genome assembly #2

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ghost opened this issue Jul 11, 2017 · 0 comments
Open

New issue of Markerminer with genome assembly #2

ghost opened this issue Jul 11, 2017 · 0 comments

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@ghost
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ghost commented Jul 11, 2017

Hi there,

Beside transcriptome data from 1kp (these data ran fine), however, I also have a nuclear genome sequence accessed here (paper: http://www.cell.com/molecular-plant/abstract/S1674-2052(17)30103-X; data: http://www.plantkingdomgdb.com/tea_tree/). When I run MarkerMiner with modified names as "DAT1-Camellia.fa" for this genome sequence, and I got error as below:
Traceback (most recent call last):
File "/apps/gcc/5.2.0/markerminer/20150710/bin/markerMiner.py", line 156, in
prefix=speciesCode + "_", suffix="", minLen=minTranscriptLen)
File "/apps/gcc/5.2.0/markerminer/20150710/bin/helpers.py", line 48, in renameFastaHeaders
for fastaRec in SeqIO.parse(fastaFile, "fasta"):
File "/apps/gcc/5.2.0/python/2.7.10/lib/python2.7/site-packages/Bio/SeqIO/init.py", line 600, in parse
for r in i:
File "/apps/gcc/5.2.0/python/2.7.10/lib/python2.7/site-packages/Bio/SeqIO/FastaIO.py", line 122, in FastaIterator
for title, sequence in SimpleFastaParser(handle):
File "/apps/gcc/5.2.0/python/2.7.10/lib/python2.7/site-packages/Bio/SeqIO/FastaIO.py", line 67, in SimpleFastaParser
yield title, "".join(lines).replace(" ", "").replace("\r", "")
MemoryError

My cmd is
markerMiner.py
-singleCopyReference Athaliana
-minTranscriptLen 500
-transcriptFilesDir /cactus/Theaceae
-outputDirPath /cactus/MarkMiner_results/Theaceae
-overwrite

Please help here and let me know if you need more infor.

Thank you so much!

Miao

@vivekkrish vivekkrish assigned vivekkrish and unassigned vivekkrish Jul 11, 2017
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