diff --git a/current-year/practicals/reference_genome/pt-1-read-cleaning.md b/current-year/practicals/reference_genome/pt-1-read-cleaning.md index ec22d2d..1df7d2e 100644 --- a/current-year/practicals/reference_genome/pt-1-read-cleaning.md +++ b/current-year/practicals/reference_genome/pt-1-read-cleaning.md @@ -73,6 +73,9 @@ directory): └── WHATIDID.txt ``` +> **_Note:_** +> You should actually create this directories and get this tree structure by running `tree` command inside the directory ending with `-read_cleaning`. + Being disciplined about structuring analyses is *extremely important*. It is similar to having a laboratory notebook. It will prevent you from becoming overwhelmed by having too many files, or not remembering what you did where. diff --git a/current-year/practicals/reference_genome/pt-3-prediction.md b/current-year/practicals/reference_genome/pt-3-prediction.md index eeffe81..8ac23e7 100644 --- a/current-year/practicals/reference_genome/pt-3-prediction.md +++ b/current-year/practicals/reference_genome/pt-3-prediction.md @@ -135,9 +135,10 @@ We will compare them using BLAST to known sequences from other species against t We will use [SequenceServer](https://sequenceserver.com) to run BLAST. Open [genomicscourse.sequenceserver.com](https://genomicscourse.sequenceserver.com) in your browser, paste the [example rice and honeybee protein sequences](predictions.fa) in the textbox and click on the 'BLAST' button to run a BLAST search. *THIS WILL TAKE A MINUTE -OR TWO*. Alternatively, just use the results of the +OR TWO*. + > **_Question:_** > Look at the BLAST results: > * Do any of the gene predictions have significant similarity to known