diff --git a/tests/drivers/spi/spi_loopback/boards/nrf_at_1mhz.overlay b/tests/drivers/spi/spi_loopback/boards/nrf_at_1mhz.overlay
new file mode 100644
index 000000000000..b8dd48ef2cca
--- /dev/null
+++ b/tests/drivers/spi/spi_loopback/boards/nrf_at_1mhz.overlay
@@ -0,0 +1,14 @@
+/*
+ * Copyright (c) 2025 Nordic Semiconductor ASA
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/* Note: 1 MHz is too low for fast SPI instance on some nRF platforms.
+ * In such case, use slow instance instead.
+ * This overlay is for negative test.
+ */
+
+&dut_fast {
+	spi-max-frequency = <DT_FREQ_M(1)>;
+};
diff --git a/tests/drivers/spi/spi_loopback/boards/nrf_at_2mhz.overlay b/tests/drivers/spi/spi_loopback/boards/nrf_at_2mhz.overlay
new file mode 100644
index 000000000000..74fbb3a02052
--- /dev/null
+++ b/tests/drivers/spi/spi_loopback/boards/nrf_at_2mhz.overlay
@@ -0,0 +1,14 @@
+/*
+ * Copyright (c) 2025 Nordic Semiconductor ASA
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/* Note: 2 MHz is too low for fast SPI instance on some nRF platforms.
+ * In such case, use slow instance instead.
+ * This overlay is for negative test.
+ */
+
+&dut_fast {
+	spi-max-frequency = <DT_FREQ_M(2)>;
+};
diff --git a/tests/drivers/spi/spi_loopback/testcase.yaml b/tests/drivers/spi/spi_loopback/testcase.yaml
index 2870bf06e804..e5be506cc7fe 100644
--- a/tests/drivers/spi/spi_loopback/testcase.yaml
+++ b/tests/drivers/spi/spi_loopback/testcase.yaml
@@ -232,6 +232,18 @@ tests:
     filter: CONFIG_DT_HAS_NXP_FLEXIO_ENABLED and
             CONFIG_DT_HAS_NXP_FLEXIO_SPI_ENABLED
     platform_allow: mimxrt1040_evk
+  drivers.spi.nrf54h_fast_2mhz:
+    extra_args:
+      - DTC_OVERLAY_FILE="boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay"
+      - EXTRA_DTC_OVERLAY_FILE="boards/nrf_at_2mhz.overlay"
+    platform_allow:
+      - nrf54h20dk/nrf54h20/cpuapp
+    harness: console
+    harness_config:
+      fixture: spi_loopback
+      type: one_line
+      regex:
+        - "Failed to initialize nrfx driver"
   drivers.spi.nrf54h_fast_8mhz:
     extra_args: DTC_OVERLAY_FILE="boards/nrf54h20dk_nrf54h20_cpuapp_fast.overlay"
     platform_allow:
@@ -248,6 +260,17 @@ tests:
       - EXTRA_DTC_OVERLAY_FILE="boards/nrf_at_32mhz.overlay"
     platform_allow:
       - nrf54h20dk/nrf54h20/cpuapp
+  drivers.spi.nrf54l_1mhz:
+    extra_args: EXTRA_DTC_OVERLAY_FILE="boards/nrf_at_1mhz.overlay"
+    platform_allow:
+      - nrf54l15dk/nrf54l15/cpuapp
+      - nrf54l20pdk/nrf54l20/cpuapp
+    harness: console
+    harness_config:
+      fixture: spi_loopback
+      type: one_line
+      regex:
+        - "Failed to initialize nrfx driver"
   drivers.spi.nrf54l_8mhz:
     extra_args: EXTRA_DTC_OVERLAY_FILE="boards/nrf_at_8mhz.overlay"
     platform_allow: