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LSMBO Galaxy tools

This repository contains a list of proteomic tools for Galaxy. Note that each tool depends on the LsmboFunctions.pm module.

  • Annotation Explorer: Retrieve GO terms and ancestors list
  • Fasta36 wrapper: Protein sequence comparaison
  • EaseDB: Generates a taxonomy-centered database for DAVID's EASE software
  • Fasta toolbox: Generation of Fasta files
  • Kegg extraction: Get Kegg maps from Uniprot entries or Kegg identifiers
  • MS Blast: Run NCBI blastp and generate an Excel output
  • MS Merge: Merges mgf files together
  • Protein/gene data: Extraction of protein information
  • Protein list comparator: Transforms an Excel table to compare protein's information per sample
  • RefSeq assembly to Fasta: Generation of a Fasta file based on RefSeq assemblies
  • Unicity checker: Checks peptide unicity
  • LsmboFunctions.pm: contains global methods

Technical questions

The tools have been developped and tested on a CentOS 8 with Perl v5.26. Most of the dependencies are listed in the file LsmboFunctions.pm:

  • General dependencies: Archive::Zip, Data::Dumper, File::Basename, File::Copy, File::Slurp, JSON::XS, List::MoreUtils, List::Util, LWP::Simple, LWP::UserAgent, Number::Bytes::Human, POSIX, Spreadsheet::ParseExcel::Utility, Spreadsheet::ParseXLSX, Excel::Template::XLSX
  • Specific dependencies for Annotation Explorer: IO::Uncompress::Gunzip, Spreadsheet::Reader::ExcelXML, URI::Escape
  • Specific dependencies for EaseDB: File::Path
  • Specific dependencies for Fasta36 wrapper: Parallel::Loops, Scalar::Util
  • Specific dependencies for Fasta Toolbox: Archive::Zip::MemberRead
  • Specific dependencies for Kegg extraction: DBI, Image::Size, MIME::Base64, Scalar::Util, SVG, XML::Simple
  • Specific dependencies for RefSeq assembly to Fasta: Mail::Sendmail

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