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Add section describing composite transcriptomes
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Matt Shirley committed May 28, 2024
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3 changes: 2 additions & 1 deletion .gitignore
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10 changes: 10 additions & 0 deletions docsource/running.rst
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.. command-output:: pisces run --help

The (``-t, --sample-type``) flag is used to specify the species name,
which will select the index files ``salmon quant`` uses to quantify transcript expression from the selected
set of fastq files. The species name is the top level key in the :doc:`config` file. If the ``--sample-type``
defines a transcriptome composed of transcripts from multiple species/assemblies (GTF/FASTA pairs), the
transcript expression will be quantified using a single combined index with transcript abundance jointly
calculated on all transcripts from the composite transcriptome. The ``pisces summarize-expression`` command will separate transcript and gene
expression estimantes (counts and TPM) into separate output tables for each species/assembly and re-scale TPM values as if the
transcript and gene expression were estimated independently. This method ensures that sequence reads which map ambiguously
between similar transcripts will be assigned to the most likely transcript during quantification.

.. _submit_example:

pisces submit
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