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clarify MCL stage moves in sample space coords
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ieivanov committed Mar 6, 2025
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Expand Up @@ -38,6 +38,8 @@ lf_slice_settings:
# steps in sample space. We can achieve this by using (300 nm)*1.4/2 = 210 nm
# steps of the mirror due to the 1.4x magnification of the remote volume and the
# fact that the optical path length is extended by twice the mirror's motion.
# The MCL Piezo stage is programmed to move in sample-space coordinates, i.e.
# (1 um)*1.4/2 = 0.7 um for every 1 um in sample space.
#
# Here we choose to oversample the data to match the light-sheet axial
# sampling. After deskewing and 3x binning, the light-sheet samples are spaced
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