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Experiment with setting the dcov property to prevent loading of massive
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shards on high coverage alignments. This should limit the number of
reads loaded in a single shard to 25k
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pauldwolfe committed Feb 15, 2019
1 parent c61393c commit c4e071a
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1 change: 1 addition & 0 deletions QScripts/BaseRecalibration.scala
Original file line number Diff line number Diff line change
Expand Up @@ -44,6 +44,7 @@ class BaseRecalibration extends QScript {

baseRecalibrator.scatterCount = numScatters
baseRecalibrator.nct = numCPUThreads
baseRecalibrator.dcov = 25000

// KODU: Apply the recalibration to the sequence data
printReads.input_file :+= bamFile
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1 change: 1 addition & 0 deletions QScripts/IndelRealignment.scala
Original file line number Diff line number Diff line change
Expand Up @@ -48,6 +48,7 @@ class IndelRealignment extends QScript {
indelRealigner.targetIntervals = targetCreator.out
indelRealigner.input_file +:= bamFile
indelRealigner.scatterCount = numScatters
indelRealigner.dcov = 25000

indelRealigner.out = swapExt(bamFile, "bam", "realigned.bam")

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