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Ampliseq digests primers #74

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iqbal-lab opened this issue Jun 21, 2022 · 0 comments
Open

Ampliseq digests primers #74

iqbal-lab opened this issue Jun 21, 2022 · 0 comments

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@iqbal-lab
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Looking through the protocol for Ampliseq it seems like the primers are being digested during the Library prep stage, after PCR. To quote the Ampliseq documentation: "Proprietary primer modifications allow the removal of primer sequences during library preparation for efficient target assessment during sequencing." (https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/ampliseq-for-illumina/ampliseq-for-illumina-custom-and-community-panels-reference-guide-1000000036408-09.pdf)

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