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Replace original filtering with ensembl canonical tag #134

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kkuret opened this issue Mar 24, 2025 · 1 comment
Open

Replace original filtering with ensembl canonical tag #134

kkuret opened this issue Mar 24, 2025 · 1 comment
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enhancement Improvement for existing functionality

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@kkuret
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kkuret commented Mar 24, 2025

Is your feature request related to a problem? Please describe

Describe the solution you'd like

Switch from basic tag and TSL- based filtering of the GTF before calling ic-count mini, to a simpler filtering that only keeps for each gene the transcript labelled as Ensembl_canonical

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@kkuret kkuret added the enhancement Improvement for existing functionality label Mar 24, 2025
@iraiosub
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iraiosub commented Mar 25, 2025

Ensembl_canonical is missing from many annotations. Updated plan is: We are instead simplifying by filtering the GTF based on either user input transcripts (one representative transcript per gene) if provided; if not provided the pipeline by default detects the longest transcript from the original input GTF (with the following hierarchy: longest CDS > longest tx (sum of all exons)) and keeps one representative (longest) transcript per gene. see issue #141

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