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R code used to clean, process, merge, and visualize data related to one of my thesis chapters.

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patriciatran/LakeMendota-PairedOmics

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Dates: 20 septembre 2019 → 31 mai 2023

Deliverable: Tran et al., 202x??

Role & Contribution: First author, project conception, grant-writing, sampling, lab work (DNA, RNA, viromics), data analyses, writing.

Status: Submitted the preprint! Paper will be submitted to journal this week!

Type: Main Thesis Chapter.

Please cite:

Patricia Q Tran, Samantha C Bachand, Benjamin Peterson, Shaomei He, Katherine McMahon, Karthik Anantharaman. "Viral impacts on microbial activity and biogeochemical cycling in a seasonally anoxic freshwater lake" bioRxiv 2023.04.19.537559; doi: https://doi.org/10.1101/2023.04.19.537559

✉️ Contact information

Name: Patricia Tran

Email: [email protected]

Overall project objective: The overall goal of this project is to understand the spatio-temporal contribution of microbial (bacteria, archaea, and viruses) in lake biogeochemical cycling, specifically in anoxic lakes.

🖥️ Data Availability

Data release: This is data from a JGI Project CSP New-Investigator written by Patricia Tran and Karthik Anantharaman (award DOI: 10.46936/10.25585/60001327) at the University of Wisconsin-Madison, obtained in 2020. This is data that I collected and generated as part of my PhD thesis in Freshwater and Marine Science at the University of Wisconsin-Madison.

The data is released to the public and is under 2 year embargo.

Item Details
JGI Proposal 506328
Number of bacterial samples 16
Number of viral samples 14
Time span July 2020 to October 2020
Location Deep Hole Lake Mendota
NCBI Project https://dataview.ncbi.nlm.nih.gov/object/PRJNA758276 (will be public upon publication)

🧬 Total Metagenomes & Viromes data link on the JGI:

https://genome.jgi.doe.gov/portal/Paivirtimeseries/Paivirtimeseries.info.html

🧬 Data Availability

The MAGs can be accessed on BioProject PRJNA758276. Under this Bioproject can be accessed all the 431 prokaryotic metagenome-assembled genomes, and the processed 16 RNA-seq samples. The phage genomes can be accessed at 10.6084/m9.figshare.22213846. (note to readers: this will be available after publications - reviewers are sent a private repo link)

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R code used to clean, process, merge, and visualize data related to one of my thesis chapters.

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